MALDI-TOFmass spectrometryas a tool for di¡erentiationof invasiveand noninvasiveStreptococcuspyogenes isolates
نویسندگان
چکیده
MALDI-TOFmass spectrometryas a tool for di¡erentiationof invasiveand noninvasiveStreptococcuspyogenes isolates Hercules Moura, Adrian R. Woolfitt, Maria G. Carvalho, Antonis Pavlopoulos, Lucia M. Teixeira, Glen A. Satten & John R. Barr Division of Laboratory Sciences, National Center for Environmental Health and Division of Bacterial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, M.S. F-50, Atlanta, GA, USA; and Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
منابع مشابه
Comparing MALDI-ToFMass Spectrometry with Molecular and Biochemical Methods in Identifying Enterococcus Faecium and Enterococcus FaecalisIsolated from Clinical Samples
Abstract Background and Objective: Enterococci are Gram-positive members of human gastrointestinal flora,in Dairy products and environment. they have emerged as important causes of opportunistic nosocomial infections in recent years. In this study we aimed to investigat and compare the efficiency of MALDI-TOFmass spectroscopy method through Biochemical and Molecular methods for detecting Enter...
متن کاملCombinationof capillaryelectrophoresis, PCRandphysiological assays in di¡erentiationof clinical strainsofStaphylococcus aureus
Combinationof capillaryelectrophoresis, PCRandphysiological assays in di¡erentiationof clinical strainsofStaphylococcus aureus Katarzyna Hrynkiewicz, Ewa Kłodzińska, Hanna Dahm, Jacek Szeliga, Marek Jackowski & Boguslaw Buszewski Department of Microbiology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń, Poland; Environmental Chemistry and Bioanalytics, Facult...
متن کاملInsufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit
While pulsed-field gel electrophoresis (PFGE) is recognized as the gold standard method for clonality analysis, MALDI-TOF MS has recently been spotlighted as an alternative tool for species identification. Herein, we compared the dendrograms of multi-drug-resistant (MDR) Acinetobacter baumannii isolates by using MALDI-TOF MS with those by using PFGE. We used direct colony and protein extraction...
متن کاملEvaluation of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in Identification of Nontuberculous Mycobacteria.
BACKGROUND/AIMS Species-level identification of nontuberculous mycobacteria (NTM) is important in making decisions about the necessity and choice of antimicrobial treatment. The reason is predictable clinical significance and the susceptibility profile of specific NTM species. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is recognized as a diagnost...
متن کاملEvaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry System for Identification of Clinical and Environmental Isolates of Burkholderia pseudomallei
Burkholderia pseudomallei is not represented in the current version of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. A total of 66 isolates of B. pseudomallei, including 30 clinical isolates collected from National Taiwan University Hospital (NTUH, n = 27) and Peking Union Medical College Hospital (PUMCH, n = 3), and 36 isola...
متن کامل